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OBJECTIVE To re-eval uate the systematic review and meta-an alysis of umeclidinium bromide and vilanterol trifenatate（UMEC/VIL）in the treatment of chronic obstructive pulmonary disease （COPD），so as to provide evidence-based basis for the treatment of COPD. METHODS Retrieved from PubMed （Medline），the Cochrane library ，Embase，CNKI，CBM，VIP and Wanfang database ，etc.，the systematic review and meta-analysis of UMEC/VIL in the treatment of COPD were collected from the inception to Apr. 2021. Two reviewers independently screened the literatures and extracted the data. AMSTAR 2 scale，PRISMA statement，and GRADE evaluation system were used to evaluate the methodological quality of the included studies ，the quality of reports and the grade of outcome indexes. The efficacy and safety of UMEC/VIL in the treatment of COPD were reported. RESULTS Six systematic reviews were finally included. The results of AMSTAR 2 scale showed that 1 study was of high quality ， 2 were of medium quality and 3 were of low quality. The score of PRISMA statement was between 21.5 and 27，and the quality of the report was relatively perfect. The results of GRADE evaluation showed that more than 60％ of the 134 outcome indicators were of medium to high quality of evidence. Comparison of effectiveness and safety showed that UMEC/VIL was superior to placebo ， unilateral bronchodilator and salmeterol fluticasone in improving forced expiratory volume in one second （FEV1）trough，FEV1 peak and forced vital capacity （FVC）. In the improvement of transition dyspnea index （TDI），SGRQ and SOBDA scores ，UMEC/ VIL was better than placebo ，unilateral bronchodilator and fluticasone propionate/salme terol. The adverse reaction rate ，acute exacerbation rate ，mortality rate ，withdrawal rate ，pneumonia rate and other negative indicators of UMEC/VIL were not inferior to placebo，unilateral bronchodilator and fluticasone propionate/salme terol. CONCLUSIONS Compared with placebo and unilateral bronchodilator，UMEC/VIL can significantly improve lung function ，symptoms and quality of life ，and has non-inferior effect for negative indicators. Compared with β2 adrenoceptor agonists combined with glucocorticoid ，UMEC/VIL can improve lung function of COPD patients ，but they are similar in other aspects.
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Objective To evaluate the efficacy and clinical significance of partial laparoscopic resection of renal pedicle without renal pedicle vascular resection in the treatment of low renal complex tumors.Methods Retrospective analysis of retroperitoneal laparoscopic renal artery clamping without partial nephrectomy in the treatment of the patients with low-grade complex renal tumors in 10 cases(observation group) and the clinical data of retroperitoneal laparoscopic renal pedicle occlusion of renal vascular resection in 10 patients (control group) were analyzed.The amount of bleeding,operation time,the average hospitalization time,drainage tube placement after operation,the hemoglobin (Hb),serum creatinine(Ccr) and glomerular filtration rate(GFR) changes were observed in the two groups.Results The operation time,bleeding volume,postoperative drainage tube placed time,postoperative hospitalization time of the observation group were (103.0 ± 42.7) min,(100.0 ± 70.4) mL,(3.5 ± 1.2) d,(5.0 ± 1.2) d,respectively,which of the control group were (129.2 ± 57.5) min,(89.2 ± 9.5) mL,(6.5 ± 9.7) d,(6.3 ± 3.4) d,respectively,there were statistically significant differences between the two groups (t =4.23,3.23,1.57,4.24,all P ＜0.05).Before operation,the Hb,Ccr and GFR between the two groups had no statistically significant differences(all P ＜0.05).After operation,the Hb,Ccr and GFR of the control group were (85.7 ± 18.2) g/L,(77.9 ± 22.1) μmol/L,(61.4 ± 50.9) mL · min-1 · 1.73 (m2)-1,respectively,which of the observation group were (95.6 ± 13.5) g/L,(70.2 ± 10.5) μmol/L,(85.5 ± 5.1) mL · min-1 · 1.73 (m2)-1,respectively,there were statistically significant differences between the two groups(t =9.34,7.44,7.34,all P ＜0.05).Conclusion Retroperitoneal laparoscopic renal partial nephrectomy without blocking renal pedicle vessels is a safe and effective method for the treatment of low complexity renal tumors.It has advantages of simple operation and is beneficial to the recovery of patients'function.
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Objective To analyze the clinical effect of minimally invasive percutaneous nephrolithotomy titanium laser lithotripsy(MPCNL)on upper ureteral calculi.Methods According to digital table,68 patients with upper ureteral calculi were randomly divided into the study group and control group,cach group in 34 cases.The study group received the MPCNL treatment,the control group received extracorporeal shock wave lithotripsy(ESWL)therapy. The therapeutic effects were compared.Results The success rate of gravel,stone clearance rates of the study group were 91 .1 8% and 91 .1 8%,which were higher than 70.59%,67.65% of the control group,there were significant differences between the two groups(χ2 =4.660,5.757,P =0.031 ,0.0164).Conclusion Percutaneous nerve titanium laser lithotripsy for upper ureteral stones can achieve good therapeutic effect.
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The objective of this study was to construct an improved thioredoxin fusion protein expression system, and express the cathelicidin-derived peptide, Lf-CATH2. The improved fusion vector Lf-CATH2-pET32α(-TS) was successfully constructed by firstly deleting the thrombin site and S tag from the pET-32α vector, then inserting the Lf-CATH2 plus a thrombin site instead. Afterwards, Lf-CATH2 was expressed in Escherichia coli as fusion protein. After the cleavage by thrombin, Lf-CATH2 was released and subsequently separated using affinity chromatography. The antimicrobial activity of purified Lf-CATH2 was also examined. The improved expression vector significantly increased enzyme cleavage efficiency by 37%, and Lf-CATH2 could be expressed in high yield and maintain the biological activity. This novel thioredoxin fusion protein expression system enables a quick production of high-yield bioactive cationic peptides like cathelicidins.
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Cathelicidins , Chromatography, Affinity , Escherichia coli , Genetic Vectors , Recombinant Fusion Proteins , Thioredoxins , GeneticsABSTRACT
OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of Protocatechuic acid and Protocatechuic aldehyde in Fuxuekang granules. METHODS: The chromatography was carried on Achirom Bond-1 C18 column(250 mm?4.6 mm,5 ?m). The mobile phase was composed of methanol - water (20∶80,pH=2.80 adjusted by glacial acetic acid) at a flow rate of 1.0 mL?min-1. The detection wavelengths were programmed at 256 nm for Protocatechuic acid and at 280 nm for Protocatechuic aldehyde. RESULTS: The linear ranges for Protocatechuic acid and Protocatechuic aldehyde were 5.49～73.22 ?g?mL-1(r=0.999 9) and 4.81～64.13 ?g?mL-1(r=0.999 9), respectively, and their average recoveries were 96.86%(RSD=2.52%,n=9) and 97.55%(RSD=3.82%,n=9) respectively. CONCLUSION: The method is simple, sensitive, and reproducible, and it is applicable for the quality control of Fuxuekang granules.